HUVEC were treated without (control) or with EtOH (25mM) for 24h. (a) QRTPCR analysis of angiopoietin-1 (Ang1) and Tie2 mRNA expression. Data are normalized to GAPDH, mean ±SEM, n=3. (b) Western blot analysis of Ang1 and Tie2 protein levels in control and ethanol treated endothelial cells. Representative blots shown, together with cumulative data, mean ± SEM, n=3.