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. Author manuscript; available in PMC: 2009 Nov 25.
Published in final edited form as: Cardiovasc Res. 2008 Apr 30;79(2):313–321. doi: 10.1093/cvr/cvn108

Figure 4. Ethanol stimulates Notch signaling in HUVEC.

Figure 4

Endothelial cells were treated without (control) or with EtOH (25mM) for 24h. (a) QRTPCR analysis of Notch 1 and 4 receptors and Notch target gene hrt-1, 2 and 3. Data were normalized to GAPDH and represent the mean ± SEM values from three independent experiments. (b) Representative Western blots of Notch 1 and 4 IC showing increase in protein expression following ethanol treatment. (c) The effect of ethanol on CBF-1/RBP-Jk promoter activity determined by luciferase assay as described in Methods. Data represent the mean ± SEM, n=3. *P<0.05 vs control.