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. Author manuscript; available in PMC: 2009 Nov 25.

Published in final edited form as: Cardiovasc Res. 2008 Apr 30;79(2):313–321. doi: 10.1093/cvr/cvn108

HUVEC mock transfected (p7pcmv) or transfected with RPMS-1 (an Epstein Barr virus-encoded gene product that binds CBF-1) were treated with or without EtOH (25mM, 24 h) before their network formation on Matrigel and Ang1, Tie2 expression were determined. (a) Network formation data (cumulative data, n=3). (b) QRTPCR analysis of Ang1 and Tie2 mRNA. Data were normalized to GAPDH and represent the mean ± SEM values from three independent experiments. *P<0.05 vs mock transfected, ^#p<0.05 vs EtOH treated mock transfected. (c) siRNA-directed knockdown of Ang1 and Tie2 inhibits ethanol-stimulated network formation. Representative Western blots for Angiopoietin 1 (Ang1) and Tie2 protein following respective siRNA knockdown. HUVEC transfected with scrambled RNA (control) or with an siRNA targeted to Ang1 or Tie2 were treated with or without ethanol (EtOH, 25 mM, 24 h) before their network formation on Matrigel was determined. *P<0.05 vs control, ^#p<0.05 vs EtOH treated control.

HUVEC mock transfected (p7pcmv) or transfected with RPMS-1 (an Epstein Barr virus-encoded gene product that binds CBF-1) were treated with or without EtOH (25mM, 24 h) before their network formation on Matrigel and Ang1, Tie2 expression were determined. (a) Network formation data (cumulative data, n=3). (b) QRTPCR analysis of Ang1 and Tie2 mRNA. Data were normalized to GAPDH and represent the mean ± SEM values from three independent experiments. *P<0.05 vs mock transfected, ^#p<0.05 vs EtOH treated mock transfected. (c) siRNA-directed knockdown of Ang1 and Tie2 inhibits ethanol-stimulated network formation. Representative Western blots for Angiopoietin 1 (Ang1) and Tie2 protein following respective siRNA knockdown. HUVEC transfected with scrambled RNA (control) or with an siRNA targeted to Ang1 or Tie2 were treated with or without ethanol (EtOH, 25 mM, 24 h) before their network formation on Matrigel was determined. *P<0.05 vs control, ^#p<0.05 vs EtOH treated control.