Effect of minocycline, doxycycline, and deferoxamine on ferritin induction by ferrous sulfate. Bars represent mean ferritin band density (± S.E.M, n = 6-9/condition) in mixed neuron-glia cultures harvested after 24 hour incubation with ferrous sulfate (Fe) 10 μM alone or with 30 μM minocycline (MN), doxycycline (DX), or deferoxamine (DFO). Sham wash (SW) cultures were subjected to washes and medium exchange only. Representative immunoblot was immunstained with anti-horse spleen ferritin and anti-actin (gel loading control). **P<0.01. v. ferrous sulfate alone.