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. Author manuscript; available in PMC: 2009 Nov 25.
Published in final edited form as: Drug Discov Ther. 2008 Oct;2(5):289–295.

Figure 3.

Figure 3

The effect of TIEG2 on ethanol-induced cell death. (A) Compare the expression of TIEG2 and MAO B catalytic activity in TIEG2/pcDNA stably transfected SH-SY5Y cell lines. (a) Western Blot analysis of the expression of TIEG2 in TIEG2/pcDNA overexpressed cell lines. (b) MAO B catalytic activity is about 2 times higher in TIEG2 stably transfected cells than pcDNA stably transfected cells. Values are expressed as means ± S.D. of at least three independent experiments._*P < 0.02 (paired t-test). (B) Effects of ethanol, ethanol plus deprenyl and TIEG2 on cell survival rates. pcDNA3.1 stably transfected cells or TIEG2 stably transfected cells were treated with 75 mM ethanol with/without 0.25 nM deprenyl for three days. Then the cell viability was determined by MTT assay. (C) Effects of different dosage of deprenyl on MAO B catalytic activity and the protection of ethanol induced cell death in TIEG2-stably transfected SH-SY5Y cell line. Cells were treated with or without 75 mM ethanol in the conjunction with 0.125, 0.25, 0.5 or 1 nM of deprenyl as indicated in the figure for three days. Then (a) the MAO B catalytic activity or (b) cell viability (MTT assay) was determined. All data are presented as the mean ± S.D. of at least three independent experiments. Controls were untreated cells (0 mM of ethanol in B and 0 nM of deprenyl in C) which were taken as 100%. *P < 0.05 versus control cells and # < 0.05 versus cells-stably expressing TIEG (one-way ANOVA followed by a post hoc Bonferroni’s t-test).