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. Author manuscript; available in PMC: 2009 Nov 25.

Published in final edited form as: Clin Cancer Res. 2008 Oct 1;14(19):5995–6004. doi: 10.1158/1078-0432.CCR-08-0291

A and B. Histological analysis of orthotopic pancreatic tumors derived from the T-HPNE.SHH cell line. Tumor sections were stained for the marker SMA to determine if there was a difference in the amount of SMA+ desmoplasia in the SHH expressing tumor cells. Positive staining for SMA is indicated in brown. (A) 40× magnification of peripheral sections of T-HPNE and T-HPNE.SHH pancreatic tumors representing the large desmoplastic reaction in the periphery of the T-HPNE.SHH orthotopic tumors (note black arrows). Infiltrating SMA+ cells are also indicated with a black arrow. (B). 200× magnification. Immunohistochemical analysis using SMA to stain for myofibroblasts in the T-HPNE and T-HPNE.SHH tumor sections. Positive staining is indicated in brown and SMA+ cells are shown with the black arrows.

C. Morphometric analysis of desmoplasia in T-HPNE vs. T-HPNE.SHH orthotopic tumors. Analysis was done by quantifying SMA+ areas on the tumor sections relative to the total cellular content, and expressing the results as a percentage: SMA+ desmoplasia/total area ×100. Tumors derived from the T-HPNE.SHH cell line showed significantly more desmoplasia than the tumors derived from the T-HPNE cells (n=13/ group).

A and B. Histological analysis of orthotopic pancreatic tumors derived from the T-HPNE.SHH cell line. Tumor sections were stained for the marker SMA to determine if there was a difference in the amount of SMA+ desmoplasia in the SHH expressing tumor cells. Positive staining for SMA is indicated in brown. (A) 40× magnification of peripheral sections of T-HPNE and T-HPNE.SHH pancreatic tumors representing the large desmoplastic reaction in the periphery of the T-HPNE.SHH orthotopic tumors (note black arrows). Infiltrating SMA+ cells are also indicated with a black arrow. (B). 200× magnification. Immunohistochemical analysis using SMA to stain for myofibroblasts in the T-HPNE and T-HPNE.SHH tumor sections. Positive staining is indicated in brown and SMA+ cells are shown with the black arrows.

C. Morphometric analysis of desmoplasia in T-HPNE vs. T-HPNE.SHH orthotopic tumors. Analysis was done by quantifying SMA+ areas on the tumor sections relative to the total cellular content, and expressing the results as a percentage: SMA+ desmoplasia/total area ×100. Tumors derived from the T-HPNE.SHH cell line showed significantly more desmoplasia than the tumors derived from the T-HPNE cells (n=13/ group).