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. Author manuscript; available in PMC: 2009 Nov 25.
Published in final edited form as: Clin Cancer Res. 2008 Oct 1;14(19):5995–6004. doi: 10.1158/1078-0432.CCR-08-0291

Figure 5. SHH enhances pancreatic stellate cell proliferation and differentiation and enhances pancreatic myofibroblast invasion.

Figure 5

Figure 5

A. Confocal microscopy of T-HPNE and T-HPNE.SHH tumors (as indicated) using anti-human ribonuclear protein (blue) and anti-SMA (green). DIC plus overlay shows that in the T-HPNE.SHH tumors, there are cells positive for SMA but not human ribonuclear protein, which indicates that the majority of the myofibroblasts are of murine origin. Confocal analysis of anti-ribonuclear protein (RNP) or SMA expression on Capan-2 control tumors and tumors treated with the 5E1 inhibitor (as indicated). The anti-human ribonuclear protein is shown in blue and SMA is in green. In the 5E1-teated Capan-2 tumors, isolated SMA+ cells can be identified as green in the overlay.

B. Serial sections showing SMA and Cyclin D1 staining of SMA+ myofibroblasts in Capan-2 tumors (brown stain).

C. Real time PCR analysis on markers of mesenchymal and epithelial differentiation performed on pancreatic stellate cells after 24 hour stimulation with recombinant human SHH at either 1ug/ml or 10ug/ml. SHH stimulation increased the expression of Desmin, Vimentin and Smooth muscle actin and decreased the expression of Cytokeratin 19.