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. Author manuscript; available in PMC: 2009 Nov 25.
Published in final edited form as: Stem Cells. 2009 May;27(5):1130–1141. doi: 10.1002/stem.36

Figure 5.

Figure 5

Loss of canonical signaling diminishes the multipotent progenitor pool. AHPs were transduced with Axin to specifically interfere with intracellular canonical signaling. Transduced cells were proliferated for 1 or 4 weeks, differentiated and labeled, and individually scored for expression of either Tuj1 (immature neurons) or GFAP (glia). (A): Bulk properties of axin-transduced AHPs. Axin significantly increased the fraction of progenitors differentiating into neuron (Tuj1; black bars) without affecting the glial (GFAP; gray bars) fraction, and decreased the fraction remaining UD (open bars). (B, C): Clonal analysis. Axin overexpression equally reduced colony size at 1 (plain bars) and 4 weeks (hatched bars), but significantly reduced multipotency after 4 weeks of proliferation, only. Values are means ± s.e.m., asterisks indicate significant differences from control at p ≤ .05 by two way ANOVA with Tukey post hoc test. Abbreviations: GFAP, glial fibrillary acidic protein; UD, undifferentiated.