Table 1.
Frequency of repopulating units in rapidly adherent, not rapidly adherent, and unsorted murine keratinocytes over time.1
| Number of GFP+ cells2 | 3 weeks | 5 weeks | 7 weeks | 9 weeks | |
|---|---|---|---|---|---|
| Rapidly Adherent Cells | 1,900 | 0/1 | 0/1 | 0/1 | 0/1 |
| 7,500 | 0/7 | 0/7 | 0/7 | 0/7 | |
| 30,000 | 5/9 | 4/9 | 4/9 | 4/9 | |
| 60,000 | 9/10 | 8/10 | 5/10 | 5/10 | |
| 120,000 | 8/10 | 7/10 | 7/10 | 7/10 | |
| 240,000 | 5/5 | 5/5 | 5/5 | 5/5 | |
| Repopulating Unit Frequency | 1 in 47,501 | 1 in 63,657 | 1 in 81,635 | 1 in 81,635 | |
| (± Standard Error) | (37,476–60,209) | (50,236–80,665) | (64,151–103,884) | (64,151–103,884) | |
| (95% Confidence Interval) | (29,848–75,596) | (40,021–101,253) | (50,900–130,929) | (50,900–130,929) | |
| Not rapidly Adherent Cells | 7,500 | 0/3 | 0/3 | 0/3 | 0/3 |
| 30,000 | 8/9 | 6/9 | 5/9 | 5/9 | |
| 60,000 | 9/10 | 9/10 | 8/10 | 8/10 | |
| 120,000 | 7/7 | 7/7 | 7/7 | 7/7 | |
| 240,000 | 5/5 | 5/5 | 5/5 | 5/5 | |
| Repopulating Unit Frequency | 1 in 21,094 | 1 in 27,630 | 1 in 36,196 | 1 in 36,196 | |
| (± Standard Error) | (15,888–28,006) | (21,147–36,100) | (27,962–46,856) | (27,962–46,856) | |
| (95% Confidence Interval) | (12,103–36,765) | (16,360–46,664) | (21,825–60,031) | (21,825–60,031) | |
| Unsorted Cells | 30,000 | 8/10 | 7/10 | 6/10 | 6/10 |
| Repopulating Unit Frequency | 1 in 18,640 | 1 in 24,918 | 1 in 32,741 | 1 in 32,741 | |
| (± Standard Error) | (12,583–27,613) | (16,683–37,218) | (21,455–49,964) | (21,455–49,964) | |
| (95% Confidence Interval) | (8,629–40,627) | (11,350–54,704) | (14,299–74,969) | (14,299–74,969) | |
1
Adapted from [17]
2
Added to chamber with 2 million GFP-negative cells.