Skip to main content
. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Arch Biochem Biophys. 2009 Sep 3;491(1-2):96–105. doi: 10.1016/j.abb.2009.08.022

Fig. 4.

Fig. 4

EPR spectra of peroxynitrite-treated CBS. Fe(III)CBS (120 μM) was mixed with increasing concentrations of peroxynitrite (0–8 mM) in phosphate buffer (0.1 M, pH 7.4). After 5 min at 37 °C, samples were frozen in liquid nitrogen. The EPR spectra were recorded at 10 K, 1 mW microwave power, 2 × 104 receiver gain, 12.78 G modulation amplitude, 9.393 GHz microwave frequency, and 100 kHz modulation frequency using 1024 data points. Each spectrum represents a single scan. The asterisk denotes a signal present in the cavity. The line markers are at g = 6, 4, 2.5, 2.3, and 1.86, respectively. ROA, reverse order of addition control.