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. Author manuscript; available in PMC: 2010 Nov 1.

Published in final edited form as: Arch Biochem Biophys. 2009 Sep 3;491(1-2):96–105. doi: 10.1016/j.abb.2009.08.022

Table 2.

Monoisotopic molecular mass/charge ratios of nitrated peptides and identification of nitration sites

Peptide From-To	Theoretical ^am/z	Observed m/z	Assigned sequence	Nitrated residue
40–51	Native 1436.76	1436.77	EPLWIRPDAPSR ^b	W43 ^b
		1452.76
		1465.76
		1468.75
	Nitrated 1481.75	1481.75	EPLW_NO2IRPDAPSR ^b

197–209	Native 1486.71	1486.70	FDSPESHVGVAWR ^b	W208 ^b
		1502.72
		1515.71
		1518.72
	Nitrated 1531.70	1531.71	FDSPESHVGVAW_NO2R ^b

212–224	Native 1598.79	1598.69	NEIPNSHILDQYR	Y223
		1611.71
		1627.68
	Nitrated 1643.78	1643.69	NEIPNSHILDQY_NO2R

210–224	Native 1839.97	1839.85	LKNEIPNSHILDQYR	Y223
		1868.80
	Nitrated 1884.96	1884.85	LKNEIPNSHILDQY_NO2R

^a

The nitro group results in a mass increase of 44.99 Da.

^b

The sequence and nitration site were confirmed by MS/MS analysis.