Table 3.
Results of protein database search with MS/MS spectrum data
| (A) Sequence matched to MS/MS data generated from precursor ion m/z 1481.75. Mascot ion score = 19 (p < 0.05). | ||||
|---|---|---|---|---|
| b | AA | y | ||
| 130.05 | 1 | E | 12 | |
| 227.1 | 2 | P | 11 | 1352.71 |
| 340.19 | 3 | L | 10 | 1255.65 |
| 571.25 | 4 | WNO2 | 9 | 1142.57 |
| 684.34 | 5 | I | 8 | 911.51 |
| 840.44 | 6 | R | 7 | 798.42 |
| 937.49 | 7 | P | 6 | 642.32 |
| 1052.52 | 8 | D | 5 | 545.27 |
| 1123.55 | 9 | A | 4 | 430.24 |
| 1220.61 | 10 | P | 3 | 359.2 |
| 1307.64 | 11 | S | 2 | 262.15 |
| 12 | R | 1 | 175.12 | |
| (B) Sequence matched to MS/MS data generated from precursor ion m/z 1531.71. Mascot ion score = 26 (p < 0.05). | ||||
|---|---|---|---|---|
| b | AA | y | ||
| 148.08 | 1 | F | 13 | |
| 263.1 | 2 | D | 12 | 1384.62 |
| 350.13 | 3 | S | 11 | 1269.6 |
| 447.19 | 4 | P | 10 | 1182.57 |
| 576.23 | 5 | E | 9 | 1085.51 |
| 663.26 | 6 | S | 8 | 956.47 |
| 800.32 | 7 | H | 7 | 869.44 |
| 899.39 | 8 | V | 6 | 732.38 |
| 956.41 | 9 | G | 5 | 633.31 |
| 1055.48 | 10 | V | 4 | 576.29 |
| 1126.52 | 11 | A | 3 | 477.22 |
| 1357.58 | 12 | WNO2 | 2 | 406.18 |
| 13 | R | 1 | 175.12 | |
N-terminal b and C-terminal y ions are listed. Ions detected in the MS/MS spectrum are shown in bold. AA, amino acids.