Figure 4.
Bmp2 expression is necessary for calcification induced by Notch inhibition. A, Western blot analysis showed that Bmp2 siRNA specifically knocked down Bmp2 but not Bmp4. B, Sheep AVICs treated with Bmp2 siRNA and DAPT for 3 weeks had >80% less calcification than cells treated with control siRNA and DAPT (3.4 ± 1.5% vs 20.1 ± 5.7%, *P=0.02 vs. control siRNA; n=5).