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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Cancer Res. 2009 Oct 20;69(21):8275–8283. doi: 10.1158/0008-5472.CAN-09-1067

Figure 4. Inhibition of the PI3K and MAP kinase pathways suppresses proliferation in minimal media of the PTEN−/− mammary epithelial cells.

Figure 4

(A.) Percent growth of three PTEN−/− clones in minimal assay media with increasing concentration of LY294002. (B.) Percent growth of three PTEN−/− clones in minimal assay media with increasing concentration of U0126. (C.) The three PTEN−/− clones were plated in minimal assay media and allowed to recover overnight. The next day, fresh minimal assay media was added +/− 10µM LY294002 (L) or 10µM U0126 (U) for 1 hour. Western blot analysis demonstrating pAKT reduction in the cells treated with the PI3K inhibitor, LY294002, and pERK reduction in the cells treated with the MEK inhibitor, U0126.