Fig. 3. Assessment of microarray data reliability.

(A) 5 µg of total RNA from T98G glioblastoma cells treated as indicated were analyzed via northern blot to verify changes in expression for a selected group of genes (left panels). Microarray data are visualized as heat maps showing changes in expression (log₂ ratios) obtained from the microarray hybridizations according to the color scale included below. (B) T98G cells were transduced with Ad-tTA and Ad-T-dnCDK9 for 6 h. Identical sets of transduced cells were incubated with tetracycline or vehicle for 42 additional h. Cells were harvested for western and northern blot analyses as described in Fig. 1 and Fig. 2A, respectively. Northern blot analysis and corresponding microarray data are shown as in A (C) Bar graph representing the average expression change (log₂ ratio) in expression for transcripts modulated by CDK9 inhibition, which are detected by more than one probe set in the microarray. Standard deviation error bars are shown.