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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Mol Genet Metab. 2009 Jul 4;98(4):356–366. doi: 10.1016/j.ymgme.2009.06.016

Figure 1. Detection of NSDHL in whole protein extracts by Western blot analysis using a purified anti-NSDHL polyclonal antibody.

Figure 1

Approximately 10 µg of total protein from WT and Bpa1H MEFs, WT E16.5 embryo, 7 mo WT female liver and 7 mo WT female brain were resolved by PAGE, transferred to nylon membrane and probed with a polyclonal anti-NSDHL antibody at a 1:4000 dilution. Antibody binding was visualized by chemiluminescence detection (see methods). A single prominent band that migrated slightly above the 37 kD marker was detected in all samples except the Bpa1H MEF. A duplicate blot was probed with an anti-β-tubulin antibody to verify the presence of intact protein in the extracts.