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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Free Radic Biol Med. 2009 Sep 12;47(11):1539–1552. doi: 10.1016/j.freeradbiomed.2009.09.003

Table 2.

Blank plasma samples (n = 5) spiked with known amounts (2.5 ng/ml or 5 ng/ml) of iso[4]LGE2-lysoPE-HL (omit addition of PLA2 and CaCl2 during PLA2 treatment to avoid the generation of additional isoLG-lysoPE-HLs from endogenous phospholipids in plasma). Inter-day study was conducted in 3 days. Data points represent the means of duplicated determinations from a representative experiment performed on 3 separate occasions.

Nominal
Concentration
(ng/mL)		 Calculated
Concentration (ng/mL)
(mean ± SD)		 Intra-day
CV (%)		 Inter-day
CV (%)		 Accuracy
(%)
2.5 2.45 ± 0.11 2.12 4.49 −2.00
5.0 4.88 ± 0.26 3.15 5.32 −2.40