Table 1.
Primer sequences used for real-time quantitative PCR of mRNA expression in hippocampal tissue.
| Gene | Primer sequences from 5′f to 3′f | |
|---|---|---|
| GFAP | F | CTGGAGGTGGAGAGGGACAA |
| R | GGTTGGTTTCATCTTGGAGCTT | |
| Probe | TTTGCACAGGACCTCGGCACCC | |
| Murine IL-α | F | AAGGAGAGCCGGGTGACAGT |
| R | GAAACTCAGCCGTCTCTTCTTCA | |
| Probe | CAGCAACGTCAAGCAACGGGAAGATTC | |
| Murine IL-β | F | TCG CTC AGG GTC ACA AGA AA |
| R | ATCAGAGGCAAGGAGGAAACAC | |
| Probe | CATGGCACATTCTGTTCAAAGAGAGCCTG | |
| MIP-2 | F | CAAGAACATCCAGAGCTTGAGTGT |
| R | TTTTGACCGCCCTTGAGAGT | |
| Probe | CCCACTGCGCCCAGACAGAAGTCAT | |
| MHC-II | F | AGTCAGTCGCAGACGGTGTTT |
| R | GATAAGACAGCTTGTGGAAGGAATAGT | |
| Probe | TGAGACCAGCTTCTTCGTCAACCGTG | |
| MCP-1 | F | GGCTCAGCCAGATGCAGTTAA |
| R | CCTACTCATTGGGATCATCTTGCT | |
| Probe | CCCCACTCACCTGCTGCTACTCATTCA | |
| KC (CXCL1) | F | GCTAAAAGGTGTCCCCAAGTAA |
| R | TAGGACCCTCAAAAGAAATTGTA | |
| Probe | CTGCTCTGATGGCACCGTCTGGT | |
| CCR2 | TaqMan® Gene Expression Assay (ID Mm99999051_gH; Applied Biosystems) | |
| CXCR2 | F | GTCTTTCAGCATGGCTCATTAC |
| R | CGTGACCTCTTTCTCCCTGTA | |
| Probe | AGACTGTGGTATTTGAATTGATGCAGCC | |
| 18s rRNA | F | CGACCATAAACGATGCCGACT |
| R | GTGGTGCCCTTCCGTCAA | |
| Probe | CGGCGGCGTTATTCCCATGACC |