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. Author manuscript; available in PMC: 2010 Nov 24.
Published in final edited form as: Biochemistry. 2009 Nov 24;48(46):10976–10987. doi: 10.1021/bi901291c

Figure 2.

Figure 2

Effect of Cu-P (Cu (II)-1, 10-phenanthroline) treatment on the dimerization of Ste2p containing single cysteine mutations. Membrane proteins were treated with Cu-P or left untreated, followed by SDS-PAGE, then immunoblotted and probed with anti-FLAG antibody. The upper band (∼110 kDa) represents dimerized receptor and the lower band (∼55 kDa) represents monomer. A. Receptors with Cys in TM1 or TM7 were treated with Cu-P (Right panel) or were untreated (Left Panel). Receptors indicated in bold exhibited a shift in signal from monomeric to dimeric form. B. Controls with Cys-less Ste2p-FT-HT and Ste2p-FT-HT. C. V68C and S292C receptors were untreated or treated with Cu-P in the presence or absence of NEM(N-ethylmaleimide) added prior to Cu-P treatment.