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A, the structures of the azido-aminoglycosides used to construct the array: (1) Kanamycin A mimic, (2) Tobramycin mimic, (3) neamine mimic, (4) neomycin B mimic. B, the structures of the 5’-end DY547-labeled oligonucleotide mimics of the bacterial rRNA A-site. C, the Huisgen dipolar cycloaddition reaction used to anchor 1–4 onto the agarose array surface.
