. Author manuscript; available in PMC: 2009 Nov 26.

Published in final edited form as: Carbohydr Res. 2008 Aug 22;343(17):2924–2931. doi: 10.1016/j.carres.2008.08.018

Table 1.

Summary of the percentages of aminoglycosides modified at the two highest aminoglycoside concentrations in the spotting buffer and the subsequent effect on binding to 5, an oligonucleotide mimic of the bacterial A-site.

	% Modified at Highest [Aminoglycoside]^a	% Decrease in Binding 5 at Highest [Aminoglycoside]^a	% Modifed at Second Highest [Aminoglycoside]^b	% Decrease in Binding 5 at Second Highest [Aminoglycoside]^b
AAC(2’)
1	N/A	N/A	N/A	N/A
2	3	4^c	8	3^c
3	5	1^c	19	2^c
4	2	23	17	7^c
AAC(3)
1	7	0^c	42	1^c
2	17	15	58	14
3	24	5^c	42	4^c
4	16	44	53	28

For AAC(2’) and AAC(3), this concentration is 5 mM.

For AAC(2’) this concentration is 1.67 mM while for AAC(3) this concentration is 1 mM.

The fluorescence intensity for binding to 5 for the aminoglycoside incubated with a cell lysate without the resistance-causing enzyme and with the resistance-causing enzyme are within error.