Fig. 2. Immunolocalization of p47phox in PC12 cells and cultured CG and DRG neurons.
NGF-differentiated PC12 and dissociated CG and DRG neurons were immunostained with antibodies against p47phox and secretogranin II (SGII), a marker for large dense core vesicles in neuronal cell body and nerve endings. P47phox was co-localized with SGII in the cell bodies and the neurites in PC12 cells (A), CG neurons (B) and DRG neurons (C). Scale bar: 20μm. Single digital slice fluorescent images were taken under confocal microscopy.