. Author manuscript; available in PMC: 2010 Dec 3.

Published in final edited form as: Auton Neurosci. 2009 Aug 27;151(2):90–97. doi: 10.1016/j.autneu.2009.07.010

Table 1.

Antibodies for Immunohistochemical Staining

Primary antibodies
Antigen	Host species	Dilution	Source
p47^phox (R360)	Rabbit	1:300	(De Leo et al., 1996)
p22^phox (R5554)	Rabbit	1:300	(Jesaitis et al., 1990)
SGII^	Mouse	1:1000	Abcam Inc., Cambridge, MA
TH^	Mouse	1:150	Calbiochem, La Jolla, CA
NPY	Goat	1:300	Santa Cruz Biotech., Inc., Santa Cruz, CA
CGRP	Sheep	1:1000	Abcam Inc., Cambridge, MA
Secondary antibodies
Target species	Host species	Conjugated to:	Dilution

Mouse	Donkey	FITC	1:40
Rabbit	Donkey	Cy3	1:200
Goat	Donkey	FITC	1:200
Mouse	Donkey	Cy3	1:200
Rabbit	Goat	Alexa 488	1:500
Sheep	Donkey	Rhodamine Red	1:500
Rabbit	Donkey	FITC	1:500

SGII = secretogranin II; TH = tyrosine hydroxylase; NPY = Neuropeptide Y;

CGRP = calcitonin gene-related peptide; ^=monoclonal antibodies

All secondary antibodies were purchased from Jackson ImmunoResearch Laboratories, Inc., West Grove, PA, except for goat anti-rabbit Alexa 488 was purchased from Invitrogen, Inc., Carlsbad, CA