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. Author manuscript; available in PMC: 2010 Dec 31.
Published in final edited form as: J Immunol Methods. 2009 Oct 1;351(1-2):30–35. doi: 10.1016/j.jim.2009.09.008

FIGURE 2.

FIGURE 2

MPO activity (measured as absorbance @ 460 nm) is linear with respect to PMN number (0.125–4 × 106) and incubation time (5–30 mins) for PMN isolated from adult peripheral blood (Panel A) and umbilical cord blood (Panel B). MPO activity was measured at 5 (X), 10 (open squares), 15 (open circles) and 30 minutes (filled triangles) following addition of all assay reagents. Data shown are mean ± SEM for 4 separate isolations.