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. Author manuscript; available in PMC: 2010 Nov 23.
Published in final edited form as: Vaccine. 2009 Sep 26;27(50):7087–7095. doi: 10.1016/j.vaccine.2009.09.059

Figure 6. Flow cytometric analysis of unfractionated splenocytes to characterize multifunctional cellular immune responses.

Figure 6

Immune responses to Alhydrogel-absorbed EP-1043 protein and the peptide pool in CFA/IFA were characterized using ICS and flow cytometry to assess the number and phenotype of multifunctional T-lymphocytes with respect to cytokine production. The production of IFN-γ and IL-2 by CD4+ T-lymphocytes is shown in panels A and C, respectively. Data indicating a lack of IL-4 and IL-5 production is shown in panel C. Proliferation of CD4+ T-lymphocytes following epitope peptide stimulation is shown in panel D.