Table 2.
Protein Designation1 | Amino Acid Sequence | HLA-DR Antigens Bound |
---|---|---|
Env 566 | IKQFINMWQEVGKAMY | 8 |
Env 729 | QHLLQLTVWGIKQLQ | 9 |
Gag1712 | QGQMVHQAISPRTLN | 9 |
Gag 2942 | GEIYKRWIILGLNKI | 10 |
Gag 2982 | KRWIILGLNKIVRMY | 13 |
Pol 3032 | FRKYTAFTIPSINNE | 7 |
Pol 3352 | SPAIFQSSMTKILEP | 9 |
Pol 596 | WEFVNTPPLVKLWYQ | 11 |
Pol 7112 | EKVYLAWVPAHKGIG | 10 |
Pol 7122 | KVYLAWVPAHKGIGG | 10 |
Pol 758 | HSNWRAMASDFNLPP | 8 |
Pol 9152 | KTAVQMAVFIHNFKR | 8 |
Pol 9562 | QKQITKIQNFRVYYR | 12 |
Pol 6192 | AETFYVDGAANRETK | 2 |
Pol 674 | EVNIVTDSQYALGII | 3 |
Pol 874 | WAGIKQEFGIPYNPQ | 5 |
Pol 989 | GAVVIQDNSDIKVVP | 5 |
Vpu 312 | YRKILRQRKIDRLID | 7 |
Epitopes are designed based on their location within HIV viral gene products as noted in Table 1.
Immunogenicity of the individual peptides was established by immunizing H2bxd mice with peptide emulsified in CFA and measured as a function of peptide-specific IFN-γ production using an EPISPOT. Those indicated were immunogenic in mice and used for immunization controls and assays.