TABLE 3.

Room Air Respiration Values and Oxyblot Intensities for Intact Cells and Isolated Mitochondria from CTL and sPD Cybrids

	INTACT CELL RESPIRATION					ISOLATED MITOCHONDRIAL RESPIRATION
	RCR	UCR	FCCP-rot	FCCP-rot/ug cardiol		normalized to cardiolipin
						CI	CII	CI ST3/ST4	Oxyblot
C56	5.46	1.74	30.47	0.74		0.68	0.25	3.01	0.77
C62	1.81	1.08	2.35	0.05		−0.01		0.77	0.90
C64	4.30	1.53	14.45	0.62		0.95	0.99	3.61	0.84
C68	5.23	1.68	22.01	0.75		0.24	0.22	2.66	1.30
C91	4.37	1.62	41.48	1.18		0.82	1.11	2.61	0.70
mean	4.23	1.53	22.15	0.67		0.54	0.64	2.53	0.90
SEM	0.65	0.12	6.69	0.18		0.18	0.24	0.48	0.11
P59	1.05	1.00	0.00	0.00		0.00	0.00	1.03	0.67
P60	5.33	2.49	25.38	0.68		0.48	0.56	3.58	0.50
P61	4.24	1.76	13.10	0.39		0.03	0.04	1.38	1.58
P63	2.51	1.18	8.00	0.37		0.45	0.04	2.42	1.26
P65	3.45	1.36	9.88	0.25		0.29	0.32	1.80	0.74
P66	3.58	1.53	15.96	0.47		0.93	1.08	3.36	1.02
P67	4.59	1.32	21.39	0.70		0.52	0.39	4.01	0.92
mean	3.54	1.52	13.39	0.41		0.39	0.35	2.51	0.96
SEM	0.54	0.18	3.22	0.09		0.12	0.15	0.44	0.14

Abbreviations: RCR= respiratory control ratio (FCCP uncoupled/oligomycin); UCR= uncoupled control ratio (FCCP uncoupled/basal); rot=rotenone; cardiol= cardiolipin; CI= complex I; CII= complex II; ST3/ST4= state 3 (+ADP)/state 4 (−ADP); Oxyblot= lane intensity of protein carbonyls