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. Author manuscript; available in PMC: 2010 Nov 15.
Published in final edited form as: Clin Cancer Res. 2009 Oct 27;15(22):6912–6920. doi: 10.1158/1078-0432.CCR-09-1698

Figure 3.

Figure 3

MLH1 protein accumulates in MLH1+ cells during prolonged LDR-IR as a result of compromised protein degradation. A. Western blots show that the increase in MLH1 and PMS2 proteins occurs concomitantly with decrease in MSH2/MSH6 proteins in HCT116MLH1 cells. B. MLH1 protein levels in Fig. 3A were quantified with Image J. C. Increased MLH1 protein can be seen in other MLH1+ cell lines HT29, U251 and HEC59 after LDR-IR (3.1 cGy/h for 72h). D. Protein synthesis inhibitor CHX cannot change the LDR-IR induced MLH1 accumulation (2.4 cGy/h for 96 h) in HCT116MLH1 cells. Cyclin B1 serves as a positive control, whereas PMS2, MSH2 and MSH6 are the negative controls. All Western blotting assays were repeated at least twice.