Figure 2.

(A) Determination of the n-alcohol cutoff of the Gly-R-α1 S267Q mutant. Inhibition of the effects of an EC₅ concentration of glycine by various concentrations of methanol, ethanol, propanol, butanol, pentanol, hexanol, and octanol was determined in Xenopus oocytes. Values shown are the percentage change in glycine response induced by the alcohols (mean ± SEM, determined from 4 to 9 oocytes). Hexanol and octanol did not appreciably affect the glycine-gated responses of the Gly-R-α1 S267Q mutant. (B) Determination of the n-alcohol cutoff of the GABA-R-ρ1 I307S/W328A double mutant, expressed in Xenopus oocytes. The effects of an EC₁₀ concentration of GABA were potentiated by various concentrations of hexanol, octanol, decanol, and dodecanol. Values represent the percentage change in GABA response induced by the alcohols (mean ± SEM, determined from 5 to 10 oocytes). (C) Decanol potentiates the function of the doubly mutated GABA-R-ρ1 I307S/W328A receptor but not of the wild-type GABA-R-ρ1. Sample tracings of currents from voltage-clamped oocytes expressing the wild-type GABA-R-ρ1 (Upper) and GABA-R-ρ1 I307S/W328A (Lower). Currents were induced by application of an EC₁₀ concentration of GABA alone or GABA in the presence of 14 μM decanol.