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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Virus Res. 2009 Sep 9;146(1-2):97–106. doi: 10.1016/j.virusres.2009.09.005

Figure 7. Mutations m1 and m2 impair basal and p65-induced transcription of the JCV early promoter.

Figure 7

The mutation m1, which prevents p65 binding, was introduced into JCVE-CAT to create m1-JCVE-CAT. Similarly, the mutation m2, which prevents C/EBPβ LIP binding, was introduced into JCVE-CAT to create m2-JCVE-CAT. U-87 MG cells were transfected with wild-type JCVE-CAT, m1-JCVE-CAT or m2-JCVE-CAT in the presence or absence of PCMV-p65 and/or pCMV-LIP. CAT activity was normalized to wild-type JCVE-CAT alone (lane 1) and presented as a histogram. The error bars encompass one standard deviation.