The mutation m1, which prevents p65 binding, was introduced into JCVE-CAT to create m1-JCVE-CAT. Similarly, the mutation m2, which prevents C/EBPβ LIP binding, was introduced into JCVE-CAT to create m2-JCVE-CAT. U-87 MG cells were transfected with wild-type JCVE-CAT, m1-JCVE-CAT or m2-JCVE-CAT in the presence or absence of PCMV-p65 and/or pCMV-LIP. CAT activity was normalized to wild-type JCVE-CAT alone (lane 1) and presented as a histogram. The error bars encompass one standard deviation.