Methylation of the 5-LOX promoter – 5’UTR region in maturing CGC (serum-free medium) assayed at the methylation-sensitive endonuclease (AciI, BstUI, HinP1, and HpaII) sites. Data obtained in samples from 5, 10, and 14 DIV cultures are expressed as a fold change compared to the methylation level in 1 DIV cultures. Data are expressed as mean ± SEM of 4 different cell preparations and were evaluated by one-way ANOVA followed by Dunnett’s multiple comparison test. *P<0.05, **P<0.001 vs. corresponding 1 DIV.