Skip to main content
. Author manuscript; available in PMC: 2010 Dec 29.
Published in final edited form as: Neuroscience. 2009 Sep 22;164(4):1531–1537. doi: 10.1016/j.neuroscience.2009.09.039

Fig. 6.

Fig. 6

5-LOX mRNA content decreases in old (30 DIV) CGC compared to 1 DIV cultures grown both in the serum-free (B27 supplement) and serum-containing medium. Total RNA was extracted from CGC grown in a serum-free medium (B27, open bars) and a serum-supplemented medium (Serum, closed bars) at 1 and 30 DIV. 5-LOX mRNA levels were measured by real-time PCR. The results were normalized against the corresponding cyclophilin contents and are presented as units (see text). Data are expressed as mean±SEM from 3 different cell preparations and were evaluated by independent sample t-test. *** P<0.001 compared to 1 DIV.