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The sequence of the mouse 5-LOX promoter-5’UTR analyzed for methylation states with methylation-sensitive endonucleases AciI (C↓CGC), BstUI (CG↓CG), HinP1I (G↓CGC), and HpaII (C↓CGG).
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The endonuclease recognition sites are highlighted in black (two for each endonuclease). ud are primers used in real-time PCR - forward 5’-agagaaggatgcgttggaaggt-3’, reverse 5’-gactccgggcaaagtgct-3’. Position 1 indicates the translation start codon (ATG).
