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. Author manuscript; available in PMC: 2010 Dec 7.
Published in final edited form as: Virology. 2009 Sep 25;395(1):10–20. doi: 10.1016/j.virol.2009.08.041

Figure 3. Incorporation of MSH peptide into fiber41S.

Figure 3

(A) Peptide insertion sites into the G region of fiber 41S. An oligo insertion strategy was used to clone 2 restriction sites, NotI and SanDI, flanked by flexible linkers into the G region. These restriction sites were used to insert oligos encoding two MSH peptides. (B) Trimerization of fiber 41S mutants. Expression vectors carrying each of the fiber 41S mutants were transfected into 293 cells, and cell lysates were analyzed by Western blot. To resolve trimer formation, samples remained unboiled before loading the gel. Blots were probed with 4D2 anti-fiber antibody.