Table 2.
Bacterial strains and plasmids.
| Strain or plasmid | Descriptiona | Reference |
|---|---|---|
| Strains | ||
| X90 | F prime; lacIq lac′ pro′/ara Δ[lac-pro] nalA argE[am] rifr thi-1 | |
| CH12 | X90 (DE3) | (8) |
| CH22 | X90 ssrA::cat, Cmr | (39) |
| CH94 | X90 clpP::cat, Cmr | (13) |
| CH113 | X90 (DE3) ssrA::cat, Cmr | (8) |
| CH2016 | CH12 Δrna ΔslyD::kan, Kanr | (53) |
| CH4119 | CH12 prfB(FLAG) | This study |
| CH4120 | CH113 prfB(FLAG), Cmr | This study |
| Plasmids | ||
| pYbeL-PP | pET11d::ybeL(E159P), T7 expression of YbeL-PP, Ampr | (8) |
| pYbeL-EA | pET11d::ybeL(P160A), T7 expression of YbeL-EA, Ampr | (8) |
| pYbeL-PP(uag) | YbeL-PP expressed from mRNA with UAG stop codon, Ampr | This study |
| pYbeL-PP(uga) | YbeL-PP expressed from mRNA with UGA stop codon, Ampr | This study |
| pFLAG-(m)YbeL-PP | Expresses C-terminal 49 residues of YbeL-PP fused to FLAG, Ampr | This study |
| pFLAG-(m)YbeL-PP(uag) | Expresses FLAG-(m)YbeL-PP from mRNA with UAG stop codon, Ampr | This study |
| pFLAG-(m)YbeL-PP(uga) | Expresses FLAG-(m)YbeL-PP from mRNA with UGA stop codon, Ampr | This study |
| pFLAG-(m)YbeL-rrnB | Expresses FLAG-(m)YbeL-rrnB from a nonstop mRNA, Ampr | This study |
| pFLAG-(m)YbeL-EA | Expresses C-terminal 49 residues of YbeL-EA fused to FLAG, Ampr | This study |
| pET21b::pth | pET plasmid expressing His6-tagged E. coli Pth, Ampr | This study |
| pSecM′ | pFG21b::secM′, Ampr | (20) |
| pPW500 | pTrc99a derivative expressing the N-terminal domain of phage λ cI repressor (λN) from nonstop mRNA. Ampr | (13) |
| pλN-PP | pTrc99a derivative expressing λN with C-terminal Pro-Pro. Ampr | This study |
| pλN-LA | pTrc99a derivative expressing λN with C-terminal Leu-Ala. Ampr | This study |
| pCH405Δ | pACYC184 derivative containing multi-cloning site, Tetr | (20) |
| pCH450 | pACYC184 derivative encoding araC and araBAD promoter, Tetr | (9) |
| pPth | pCH410::pth, arabinose-inducible Pth expression, Tetr | This study & (9) |
| pRF-1 | pACYC184 derivative expressing RF-1 under araBAD promoter, Tetr | (9) |
| pRF-3 | pCH450::prfC, arabinose-inducible RF-3 expression, Tetr | This study |
| pRRF | pCH450::frr, arabinose-inducible RF-3 expression, Tetr | This study |
| pRF-3/RRF | pCH450::prfC-frr, arabinose-inducible RF-3 and RRF expression, Tetr | This study |
| pIF-3 | pCH450::infC, arabinose-inducible IF-3 expression, Tetr | This study |
| pSmpB•tmRNA | pCH405Δ::smpB-ssrA, constitutive expression of SmpB•tmRNA, Tetr | This study |
| ptRNA2Pro | pCH405Δ::proL constitutive expression of tRNA2Pro, Tetr | (20) |
a
Abbreviations used: Ampr, ampicillin resistant; Cmr, chloramphenicol resistant; Kanr, kanamycin resistant; Tetr, tetracycline resistant.