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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Hum Mutat. 2009 Nov;30(11):1574ā€“1582. doi: 10.1002/humu.21116

Figure 2. PCR analysis of fetus MKS-084D.

Figure 2

Multiplex PCR were performed with primers that amplify exon 13 (expected size : 647pb) and exons 28 (484pb), 29 (384pb), 30 (482pb) respectively. Both exons are amplificated with a control DNA (C+), but only exon 13 for the MKS-084D DNA confirming the deletion of exons 28 to 30. For exon 31 (687bp) and 32 (355bp) a simplex PCR is shown. MKS84D fails to amplify exon 31 but correctly amplifies exon 32. Cāˆ’ is a negative control (no DNA in reaction).