Skip to main content
. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2009 Sep 24;29(12):2153–2160. doi: 10.1161/ATVBAHA.109.197285

Figure 1. The phosphomimetic T159D mutation negatively regulated SRF-dependent SMC-specific transcription.

Figure 1

A) Schematic of SRF functional domains and phosphorylation sites tested in these studies. B) SRF was transfected into SRF −/− ES cells along with SM22, SM α-actin, or c-fos promoter/luciferase constructs. Luciferase activity was measured 24h post-transfection and expressed relative to that measured in the presence of an equal amount of empty expression vector. C) The indicated SRF variants and promoter-reporter constructs were co-transfected into ES cells. Luciferase activity after 24h was measured and is expressed relative to empty expression vector set to 1. *p<0.05 versus Wt SRF D) Western blot showing expression levels of the SRF variants in ES cells 24h post-transfection.