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Run-off sequencing results of CatHI- or EarI-digested pUC19 DNA.
The 1.8 kb CatHI or EarI-digested DNA was gel-purified and subjected to run-off sequencing. An extra adenine (A) base was added by the Taq DNA polymerase after the polymerase “runs-off” from the cleaved template. Following the extra A base, the intensity of the sequence peak signal drops to a lower level. The red arrow indicates the precise cleavage site on top or bottom strand.
