Table 1.
Glucose uptake in WT and in Igf1−/− synaptosomes
Treatments | WT | Igf1−/− | Δ | P |
---|---|---|---|---|
Control medium | 544.3 ± 24.8 | 376.0 ± 25.7 | 31% ⇓ | 0.006 |
Medium + Igf1 | 502.7 ± 53.4 | 494.3 ± 38.9 | ⇔ | 0.9 |
Whole brain synaptosome preparations were incubated with 3H-labeled 2DG (1 μCi/ml) in the absence (control medium) or presence of Igf1. After centrifugation and washing, the radioactivity absorbed by the synaptosomal pellet was measured by scintillation counting. In control incubation, 2DGU was significantly reduced in Igf1−/− synaptosomes. When Igf1 was added to the incubation, 2DGU in the Igf1−/− was equal to WT. Results are expressed as means ± SEM in fmol/mg of protein per min. WT, n = 3; Igf1−/−, n = 4.