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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Biomaterials. 2009 Oct 9;31(4):669–679. doi: 10.1016/j.biomaterials.2009.09.087

Figure 7. Melanization does not reduce biofilm vulnerability chitosan.

Figure 7

(A) The percentage of metabolic activity of C. neoformans strain B3501 melanized and nonmelanized biofilms was measured by the XTT reduction assay. Both phenotypes were exposed to various concentrations of chitosan (0.625, 1.25, 2.5, and 5 mg/mL) for 2 h; and their metabolic activities were compared to those of fungal cells incubated in PBS. (B) Chitosan disrupts melanin from cryptococcal cells. After co-incubation with chitosan, the cells were washed and incubated with MAbs 6D2-FITC-conjugated goat anti-mouse IgM and 18B7-TRITC-conjugated goat anti-mouse IgG1 stained to label the melanin and capsular polysaccharide, respectively. Scale bar, 2 μm.