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. Author manuscript; available in PMC: 2010 Nov 10.
Published in final edited form as: Biochemistry. 2009 Nov 10;48(44):10644–10653. doi: 10.1021/bi901518y

Figure 1.

Figure 1

Label transfer analysis of SufA interactions with the other Suf proteins. (A) SufA (4 μM) pre-labeled with Mts-Atf-Biotin was incubated for 1 h with 2 μM of the other Suf proteins individually or in various combinations. Lower molecular weight bands below SufB (indicated by *) were confirmed by mass spectrometry to be proteolysis products of SufB. (B) Increasing amounts of SufA pre-labeled with Mts-Atf-Biotin were incubated for 1 h with 2 μM of SufB or the SufBCD complex. After UV-light induced cross-linking, samples from (A) and (B) were separated by reducing SDS-PAGE and the location of the biotin tag was determined by immunoblot using streptavidin conjugated to horseradish peroxidase.