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EDTA inhibits cluster acquisition by SufA. 280 μM apoSufA was incubated with 470 μM ferrous ammonium sulfate and 453 μM of sodium sulfide in the absence (grey traces) or presence (red traces) of 60 μM EDTA. Changes in CD spectra were monitored over time. Arrows indicate direction of change to CD spectra within each region over time after addition of iron and sulfide.
