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. Author manuscript; available in PMC: 2010 Dec 14.
Published in final edited form as: Biotechnol Bioeng. 2009 Dec 15;104(6):1189–1196. doi: 10.1002/bit.22491

Figure 3.

Figure 3

In vitro translation of unlinearized constructs 1–17. Proteins synthesized from 35S-Met by a crude E. coli combined transcription/translation system were resolved by SDS–PAGE and detected by autoradiography. –, No DNA; 0, plasmid identical to construct 9 but lacking a T7 promoter. Marker sizes are given in kDa. A: Single gene constructs. B: Dimeric gene constructs.