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. Author manuscript; available in PMC: 2010 Dec 14.
Published in final edited form as: Biotechnol Bioeng. 2009 Dec 15;104(6):1189–1196. doi: 10.1002/bit.22491

Figure 5.

Figure 5

In vitro transcription of single and pentameric gene constructs. A: Single gene constructs 18–20 and pentameric gene constructs. All genes contain lacO/RBS and single vsv modules, but these modules are not depicted in pentameric gene constructs for simplicity. Variable modules in the constructs are in gray. B: In vitro transcription of PstI-linearized constructs. Transcripts were separated by 3.5% PAGE/urea gel and stained with toluidine blue. The products from the single gene constructs (8, 18, 3, 19, and 20) were used as markers. Note that only one of the run through markers was short enough to be resolved from the vsv-terminated marker (construct 8). Also, for constructs 21 and 22, only one of the run through products (EF-Ts followed by IF1) was short enough to be visible beneath the EF-G band (construct 22).