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. Author manuscript; available in PMC: 2010 Nov 3.

Published in final edited form as: Biochemistry. 2009 Nov 3;48(43):10416–10422. doi: 10.1021/bi901161b

Table 1.

Kinetic constants of HuPON1, G2E6, G3C9, and variants with phenyl acetate and paraoxon

	Phenyl Acetate		Paraoxon

Enzyme	k_cat (s⁻¹)	K_M (mM)	k_cat (s⁻¹)	K_M (mM)
HuPON1	970 ± 70	0.66 ± 0.09	0.4 ± 0.07	0.57 ± 0.03
H115W	---	---	4.8 ± 0.9	0.19 ± 0.006
S193A	580 ± 120	0.50 ± 0.07	0.4 ± 0.07	1.00 ± 0.2
S193G	630 ± 80	0.23 ± 0.05	0.4 ± 0.05	0.60 ± 0.03
R214Q	600 ± 50	0.48 ± 0.06	0.3 ± 0.03	0.70 ± 0.2
S193A/R214Q	1130 ± 120	0.40 ± 0.1	0.5 ± 0.08	0.70 ± 0.3

G2E6	1150 ± 70	0.80 ± 0.1	5.7 ± 0.2	0.60 ± 0.07
H115W	16 ± 1	1.70 ± 0.3	5.7 ± 0.1	0.30 ± 0.03
S193A	1000 ± 50	1.10 ± 0.2	4.0 ± 0.07	0.49 ± 0.03
R214Q	600 ± 33	0.44 ± 0.07	3.7 ± 0.08	0.55 ± 0.04

G3C9	350 ± 120	2.30 ± 0.4	0.9 ± 0.07	0.60 ± 0.2