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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Biochim Biophys Acta. 2009 Aug 27;1791(12):1190–1196. doi: 10.1016/j.bbalip.2009.08.008

Figure 1.

Figure 1

Real-time PCR analysis of hepatic lipogenic gene expression. Rat primary hepatocytes were incubated with or without insulin (100 nM) in the presence or absence of BSA (0.15%), OLA (100 μM), EPA (100 μM), or DHA (100 μM). After 24 hours of treatment, cells were lysed and total RNA isolated. (A.) Expression of SREBP-1c, (B.) SREBP-1c target genes, (C.) and known transactivators of SREBP-1c were assessed by real-time PCR. Values expressed as the fold change from vehicle + BSA control and are the mean of at least four hepatocyte preparations (n ≥ 4). * P ≤ 0.05 vs. vehicle + BSA, # P ≤ 0.05 vs. insulin + BSA.