Figure 2.

Effect of PUFA on lipid metabolism as determined by 2-¹⁴C acetate into TG and total cellular lipids. Rat primary hepatocytes were incubated with or without insulin (100 nM) in the presence or absence of BSA (0.15%), OLA (100 μM), EPA (100 μM), or DHA (100 μM). After 24 hours of treatment, cells were pulsed with 2-¹⁴C acetate (4 μCi) and 1 mM unlabelled acetate for 3 hours to allow for incorporation into cellular lipid. (A.) Hepatocyte lipids were subjected to methanol:chloroform extraction followed by separation by TLC. Individual lipid fraction bands were collected and counted. Incorporation into TG is shown as an indicator of DNL. (B.) Incorporation of 2-¹⁴C acetate into TG, PL, and FFA were added together as an indicator of effect of PUFA on incorporation into total cellular lipids. Data are expressed the fold change (disintegrations per minute normalized to protein) from BSA + vehicle and are the mean ± SEM of 4 hepatocyte preparations (n ≥ 4). * P ≤ 0.05 vs. vehicle + BSA, # P ≤ 0.05 vs. insulin + BSA