Figure 3.

EPA and DHA effectively attenuate insulin induction of 1.5 kb SREBP-1c - Luc. Hepatocytes were transfected with both full-length rat SREBP-1c promoter luciferase construct and renilla control. Following transfection, cells were incubated with or without insulin (100 nM) in the presence or absence of BSA (0.15%), OLA (100 μM), EPA (100 μM), or DHA (100 μM). After 24 hours of treatment, cells were lysed, luciferase activity measured and normalized to renilla luminescence. Data are expressed as the fold change in normalized luciferase from vehicle + BSA control and are the mean of at least five hepatocyte preparations (n ≥ 5). * P ≤ 0.05 vs. vehicle + BSA, # P ≤ 0.05 vs. insulin + BSA