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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Biochim Biophys Acta. 2009 Aug 27;1791(12):1190–1196. doi: 10.1016/j.bbalip.2009.08.008

Figure 4.

Figure 4

DHA repression of 1.5 kb SREBP-1c – Luc activity is attenuated by LXRE mutation. Hepatocytes were transfected with full-length wild-type (WT), SRE mutant, NFY mutant, or LXREx2 mutant rat SREBP-1c promoter luciferase constructs and null renilla control. Following transfection, cells were incubated with insulin (100 nM) in the presence or absence of BSA (0.15%) or DHA (100 μM). After 24 hours of treatment, cells were lysed, luciferase activity measured and normalized to renilla luminescence. Maximal insulin-induced activity in the presence of BSA for each construct is expressed as 100% activity. DHA mediated repression is expressed as the percentage of the corresponding maximal insulin induction. Data are the mean of at least four hepatocyte preparations (n ≥ 4). * P ≤ 0.05 vs. insulin + BSA for each construct